Hemoglobin (Hb)

CPT Test code: 85018

Specimen: Whole blood
Volume: Tube fill capacity
Minimum Volume: 0.5 mL
Container: Lavender-top (EDTA) tube
Collection: Invert tube 8 to 10 times immediately after tube is filled at the time of collection.
Storage Instructions: Maintain specimen at room temperature.
Causes for Rejection: Hemolysis; clotted specimen; tube not filled with minimum volume; improper labeling; transport tubes with whole blood; specimen diluted or contaminated with IV fluid; specimen drawn in any anticoagulant other than EDTA
Use: Evaluate anemia, blood loss, hydration or suspected polycythemic condition, and response to treatment
Limitations: Hyperlipemic plasma (especially Fredrickson and Lees type I and V in which chylomicronemia is present) or white count >50,000/mm3 may falsely elevate the hemoglobin result with corresponding increase in the MCH. The laboratory will attempt to correct this interference by a special technique.