Hepatitis C Virus (HCV) Antibody
CPT Test code: 86803
|Specimen:||Serum or plasma|
|Container:||Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube|
|Collection:||If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.|
|Causes for Rejection:||Non-EDTA plasma specimen; PST gel-barrier tube|
|Use:||Assess exposure to hepatitis C virus infection; test blood safety|
|Limitations:||Since as many as 90% of commercial intravenous immunoglobulins test positive for hepatitis C antibody, an artifactual positive can result briefly after transfusion.|
|Additional Information:||Following the development of sensitive and specific testing for hepatitis B, 90% of post-transfusion hepatitis is now hepatitis C. A gene product (c100) of hepatitis C virus (HCV) was isolated and an assay for anti-HCV developed. The assay detects antibody to a presumptive togavirus or flavivirus which may be an etiologic agent of non-A, non-B hepatitis (which may not be a unitary disease entity).
For blood donors, hepatitis C serology correlates with surrogate tests for non-A, non-B hepatitis (ALT and anti-HBc). Since hepatitis C serology identifies a broader group of infected individuals than surrogate testing, it reduces risk of HCV during transfusion. Studies in hemophiliacs indicate that antibody to HCV is a reliable marker of HCV.
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Dodd LG, McBride JH, Gitnick GL, Howanitz PJ, Rodgerson DO. Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in human immunoglobulins. Am J Clin Pathol. 1992 Jan; 97(1):108-113. PubMed 1728850
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Kuo G, Choo QL, Alter HJ, et al. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989 Apr 21; 244(4902):362-364. PubMed 2496467
Richards C, Holland P, Kuramoto K, Douville C, Randell R. Prevalence of antibody to hepatitis C virus in a blood donor population. Transfusion. 1991 Feb; 31(2):109-113.PubMed 1847559