Sickle Cell

Sickle Cell

CPT Test code: 81401

Specimen: Amniotic fluid or chorionic villus sample (CVS)
Volume: 10 mL amniotic fluid or 20 mg CVS and 5 mL blood per parental sample
Minimum Volume: 5 mL amniotic fluid or 10 mg CVS and 2 mL blood per parental sample.
Container: Sterile plastic conical tube or two confluent T-25 flasks and lavender-top (EDTA) tube
Special Instructions: This test is for fetal/prenatal cases and parental controls. It is not intended for routine sickle cell screening. Please call 800-345-4363 with any questions regarding selection of tests.
Collection: Do not use urine container, syringes, or tubes with rubber-stoppers. Rubber is toxic to amniocytes.
Storage Instructions: Maintain specimen at room temperature.
Causes for Rejection: Specimen frozen; specimen found not to be amniotic fluid; quantity not sufficient for analysis; improper container
Use: DNA analysis to detect mutations known to cause sickle cell anemia
Limitations: False-positive and false-negative results may occur for reasons that include genetic variants, blood transfusions, bone marrow transplantation, erroneous representation of family relationships, or contamination of a fetal sample with maternal cells.