CPT Test code: 86225;86235
Test Includes: | Anticentromere B antibodies; anti-dsDNA; antichromatin antibodies; anti-Jo-1; RNP antibodies; antiscleroderma 70 antibodies; Smith antibodies; Sjögren anti-SS-A; Sjögren anti-SS-B |
Specimen: | Serum |
Volume: | 1 mL |
Minimum Volume: | 0.5 mL (Note: This volume does not allow for repeat testing.) |
Container: | Red-top tube or gel-barrier tube |
Special Instructions: | Test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855]. |
Storage Instructions: | Maintain specimen at room temperature. |
Causes for Rejection: | Hemolysis; lipemia; gross bacterial contamination; icterus |
Use: | Detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. |
References: | Astion ML, Wener MH, Hutchinson K, “Autoantibody Testing: The Use of Immunofluorescence and Other Assays,” Clin Lab News, 2000, (7):30-4.
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