PPT™, yellow-top (ACD) tube, or lavender-top (EDTA) tube
Special Instructions:
A separate frozen specimen is required for each frozen test ordered.
Collection:
Centrifuge specimen within two hours of collection. Remove plasma, transfer plasma to a screw-cap polypropylene transport tube (not “pop top” or “snap cap”), and freeze. Specimens collected in PPT™ need to be centrifuged within two hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions:
Freeze
Causes for Rejection:
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in “pop-top” or “snap-cap” tube
Use:
Detect resistance in HIV-1 to antiretroviral drugs
Limitations:
This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account’s prevailing fee.
References:
Hirsch MS, Brun-Vézinet F, D’Aquila RT, et al. Antiretroviral drug resistance testing in adult HIV-1 infection. Recommendations of an International AIDS Society-USA panel. JAMA. 2000; 283(18):2417-2426.PubMed 10815085
